The aim of this project is to develop a novel, portable device (FORMI) based on surface-enhanced Raman
scattering (SERS)-active nanostructures and microfluidics for sensitive, simple, quick and on-site
detection of pathogenic bacteria from clinical and environmental samples.
The proposed SERS-based device for bacteria identification challenges the standard biochemical methods
in terms of simplicity, specificity and rapidity (maximum 60 s for a single SERS measurement).
In the presented FORMI device the direct SERS analysis of bacteria (even a single bacteria cell)
is performed directly from SERS-active nanostructures incorporated into a microfluidic module.
The recorded SERS data of bacteria will be categorized (assigned to a particular bacterial species)
using data analysis software based on a SERS database created for bacteria. The long-time of incubation
of bacteria will be eliminated and the total analysis including numerical analysis of the recorded
SERS data will exceed 15 minutes. In addition, we will establish:
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method of modification of SERS platform by suitable linkage molecules for selective immobilizations of antibodies
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method of gold nanoparticles synthesis and their subsequent modification with selected Raman
reporters and/or antibodies for developing the multiplex and specific (qualitative and quantitative)
analysis of different pathogens in complex fluid.
The proposed FORMI device can be introduced to International Organization for Standardization (ISO)
standards for bacteria identification, to avoid the time-consuming methods routinely used in laboratories
and, as a result, the time of analysis will be dramatically reduced.
The FORMI device will open a new path in microbiological diagnostics for sensitive, simple, quick, and on-site detection of pathogenic bacteria, including environmental and clinical microbiology (hospitals, health centres), food industry, and environmental protection.
Nanoplasmonic sensor for foodborne pathogens detection. Towards development of ISO‐SERS methodology for taxonomic affiliation of Campylobacter spp.
Evelin Witkowska, Krzysztof Niciński, Dorota Korsak, Bartłomiej Dominiak, Jacek Waluk and Agnieszka Kamińska
Journal of Biophotonics Volume 13, Issue 5, 2020, e201960227
doi: 10.1002/jbio.201960227
Surface-enhanced Raman scattering as a discrimination method of Streptococcus spp. and alternative approach for identifying capsular types of S. pneumoniae isolates
Sylwia Berus, Evelin Witkowska, Krzysztof Niciński, Ewa Sadowy, Weronika Puzia, Patrycja Ronkiewicz and Agnieszka Kamińska
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Volume 233, 5 June 2020, 118088
doi: 10.1016/j.saa.2020.118088
Photovoltaic cells as a highly efficient system for biomedical and electrochemical surface-enhanced Raman spectroscopy analysis
Krzysztof Niciński, Evelin Witkowska, Dorota Korsak, Krzysztof Noworyta, Joanna Trzcińska-Danielewicz, Agnieszka Girstun and Agnieszka Kamińska
RSC Advances 2019, 9, pp. 576–59
doi: 10.1039/C8RA08319C
Sources of variability in SERS spectra of bacteria: comprehensive analysis of interactions between selected bacteria and plasmonic nanostructures
Evelin Witkowska, Krzysztof Niciński, Dorota Korsak, Tomasz Szymborski, and Agnieszka Kamińska
Analytical and Bioanalytical Chemistry April 2019, Volume 411, Issue 10, pp. 2001–2017
doi: 10.1007/s00216-019-01609-4
Steel Wire Mesh as a Thermally Resistant SERS Substrate
Tomasz Szymborski, Evelin Witkowska, Krzysztof Niciński, Zuzanna Majka, Tomasz Krehlik, Tomiła Deskur, Katarzyna Winkler and Agnieszka Kamińska
Nanomaterials 2018, Volume 8, Issue 9, p. 663
doi: 10.3390/nano8090663
List of conferences
Number P.436251
The method for determining the presence of venereal disease pathogens.
Sylwia Berus, Beata Młynarczyk-Bonikowska, Monika Adamczyk-Popławska, Tomasz Szymborski, Evelin Witkowska-Iwan, and Agnieszka Michota-Kamińska
We have developed a novel approach for detection pathogens responsible for sexually transmitted disease (STD).
The present method is based on a SERS technique combined with chemometric analysis (PCA, PLS-DA). Since a new method enable fast (ca. 15 minutes), simple,
label-free and accurate identification of pathogens, this new invention may significantly influence the future of diagnosis STD in real clinical samples.
Number P.434300
The method of manufacturing a silicon platform for SERS measurements, silicon platform for SERS measurements
and the use of a silicon platform for SERS measurements of biological samples.
Tomasz Szymborski, Yuriy Stepanenko, Patrycja Piecyk, Krzysztof Niciński, and Agnieszka Michota-Kamińska
The first object of the invention is the method of manufacturing a silicon platform with femtosecond laser. The second object of the invention
is a silicon platform for SERS measurements. Another object of invention is the application of the platform for direct detection and/or identification
of biological or chemical samples using a surface enhanced Raman spectroscopy (SERS).
Number P.433801
The method of deposition of microorganisms or cancer cells on the SERS platform using
a dielectrophoretic effect and identification of microorganisms or cancer cells on the SERS platform.
Tomasz Szymborski, Ariadna Nowicka, Marta Czaplicka, Joanna Trzcińska-Danilewicz,
Agnieszka Girstun, and Agnieszka Michota-Kamińska
The subject of the invention is the method of deposition of microorganisms (mainly bacteria) or cancer cells with
the use of the negative dielectrophoretic effect (n-DEP). In the proposed patent application we apply an alternating
electric field between two electrodes. Main electrode is a flexible SERS platform on which the examined objects are
deposited due to dielectrophoretic force. After depositing with the use of n-DEP technique we identify bacteria/cancer
cells via surface enhanced Raman spectroscopy (SERS) technique. The invention is used in microbiological laboratories.
Number P.430767
Flexible platform for surface-enhanced Raman effect, method of preparing such a platform,
method of determining substances and/or microorganisms using such a platform, using such
a platform for direct detection and/or identification of substances and/or microorganisms,
especially bacteria and cancer cells using the technique surface enhanced Raman effect.
Marta Czaplicka, Ariadna Nowicka, Tomasz Szymborski, Aneta Kowalska, Joanna Trzcińska-Danilewicz,
Agnieszka Girstun and Agnieszka Michota-Kamińska
The goal of the invention was to develop a platform with a high enhancing factor,
high repeatability, stability, and ease of processing, e.g. by cutting and bending. The platform would
allow the application of electrical potentials (deposition of bacteria or cancer cells using
dielectrophoretic effect), and thus capable of measuring microorganisms, and especially bacteria,
using the SERS technique.
Number P.430701
Method for detecting thermotolerant bacteria of the genus Campylobacter spp. in food.
Evelin Witkowska, Krzysztof Niciński, Bartłomiej Dominiak, Dorota Korsak, Jacek Waluk and Agnieszka Michota-Kamińska
We have developed a new approach applying novel silver/photovoltaic-based (Ag/PV) SERS substrates
in the detection of foodborne bacteria, namely thermotolerant Campylobacter spp.,
in different poultry samples. The presented method of bacteria identification based on the SERS
technique challenges the standard biochemical methods in terms of simplicity, specificity
and rapidity, and thus may significantly influence the future of microbiology and medical diagnosis.
Number P.426643
Platform for surface-enhanced Raman effect, method of preparing such a platform,
method for determining substances and/or microorganisms using such a platform,
use of such a platform for direct detection and/or identification of substances
and/or microorganisms, especially bacteria, using the surface-enhanced Raman effect
technique or in combination with electrochemical measurements.
Krzysztof Niciński, Evelin Witkowska, Dorota Korsak, Joanna Trzcińska-Danilewicz, Agnieszka Girstun, Tomasz Szymborski and Agnieszka Michota-Kamińska
We have developed a SERS platform with a high enhancement factor, high repeatability
and stability that would allow for the electrical potential to be applied
(i.e. useful for spectroelectrochemical measurements) and capable of providing suitable
surface for measuring spectra of microorganisms, especially bacteria,
using the surface-enhanced Raman scattering technique (SERS). The platform can be used
for SERS measurements of various biological and organic samples. Main element of a platform
is photovoltaic cell of which bottom layer consist of metal, constituting the bottom electrode.
Upper part is covered with a layer of doped silicon equipped with a anti-reflective layer,
on the surface of which the electrode leads are located. Additionally SERS-active metallic layer
is deposited on the anti-reflective surface.
